Isoform-Specific Na,K-ATPase Alterations Precede Disuse-Induced Atrophy of Rat Soleus Muscle

Figure 3

24–72 h of hindlimb suspension specifically alters the electrogenic activity (a) and the relative mRNA (b) and protein (c) contents of the α2 Na,K-ATPase in rat soleus muscle. α1 and α2 Na,K-ATPase, white and black columns, respectively. (a) Na,K-ATPase α1 and α2 electrogenic activity computed as the difference in resting potential measured before and after blockade of the Na,K-ATPase isozymes by ouabain. (b) and (c) data normalized to the average level of expression under control conditions. Upper panels (c) show representative immunoblots. Columns show mean data from 5 to 10 different muscle samples (panel (b)) and 10 different muscle samples (panel (c)). compare to control.

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