Isoform-Specific Na,K-ATPase Alterations Precede Disuse-Induced Atrophy of Rat Soleus Muscle

Figure 4

Changes in the resting membrane potential induced by 100 nM nicotine in rat soleus muscle in control, after 24 and 72 h of hindlimb suspensions. (a) RMPs were measured in the same muscles prior to (white columns) and after 60 min of nicotine application (grey columns); 12 muscles, control, 4 muscles, after 24 h and 8 muscles after 72 h of HS; in separate muscles RMPs were measured after 30 min of 100 nM ouabain application (cross hatched columns) and in the presence of ouabain after 60 min of nicotine application (black columns). 4 muscles, control, 4 muscles, after 24 h of HS. compared to RMPs before nicotine’s addition. (b) the effect of ouabain (30 min incubation) on the RMPs of control rat soleus muscles. Columns show mean data from 4 to 6 different muscles.

(a)

(b)