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BioMed Research International
Volume 2015, Article ID 720413, 9 pages
Research Article

Isolation and Molecular Characterization of Brucella Isolates in Cattle Milk in Uganda

1College of Veterinary Medicine, Animal Resources and Bio-Security, Makerere University, P.O. Box 7062, Kampala, Uganda
2Division of Bacteriology and Food Safety, Department of Biomedical Sciences and Veterinary Public Health, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, P.O. Box 7028, 75007 Uppsala, Sweden
3College of Health Sciences, Makerere University, P.O. Box 7062, Kampala, Uganda
4Section of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, 75185 Uppsala, Sweden
5Division of Reproduction, Department of Clinical Sciences, Faculty of Veterinary, Medicine and Animal Science, Swedish University of Agricultural Sciences, P.O. Box 7054, 75007 Uppsala, Sweden

Received 1 July 2014; Revised 22 October 2014; Accepted 23 October 2014

Academic Editor: Jacques Cabaret

Copyright © 2015 Denis Rwabiita Mugizi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Brucellosis is endemic in livestock and humans in Uganda and its transmission involves a multitude of risk factors like consumption of milk from infected cattle. To shed new light on the epidemiology of brucellosis in Uganda the present study used phenotypic and molecular approaches to delineate the Brucella species, biovars, and genotypes shed in cattle milk. Brucella abortus without a biovar designation was isolated from eleven out of 207 milk samples from cattle in Uganda. These isolates had a genomic monomorphism at 16 variable number tandem repeat (VNTR) loci and showed in turn high levels of genetic variation when compared with other African strains or other B. abortus biovars from other parts of the world. This study further highlights the usefulness of MLVA as an epidemiological tool for investigation of Brucella infections.