Isolation and Molecular Characterization of Brucella Isolates in Cattle Milk in Uganda

Figure 4

Bruce-ladder multiplex PCR agarose gel picture used to confirm the Brucella species isolated. Extreme left and right lanes are for 100 bp molecular weight marker; from left to right: lanes 2, 5–13 are for DNA from isolates detected as Brucella with strong Ct values; lanes 3, 4, and 14 are for DNA from Brucella-like isolates with weak Ct values; lanes 15 and 19 are for B. suis positive control DNA; lane 16 is for RB 51 positive control DNA; lane 17 is for Rev 1 positive control DNA; and lane 18 is for PCR grade water (negative control).