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BioMed Research International
Volume 2015, Article ID 731781, 7 pages
http://dx.doi.org/10.1155/2015/731781
Research Article

Serum miR-224 Reflects Stage of Hepatocellular Carcinoma and Predicts Survival

1Department of Integrative Medicine, Fudan University Shanghai Cancer Center, 270 Dongan Road, Shanghai 200032, China
2Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China

Received 15 August 2014; Accepted 30 October 2014

Academic Editor: Mathew Coleman

Copyright © 2015 Li-ping Zhuang and Zhi-qiang Meng. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. In our previous study, we conducted a systematic screening of miRNA to identify potential serum biomarkers for predicting venous metastasis and survival in patients with hepatocellular carcinoma (HCC). miR-224 was one of the differentially expressed miRNAs. This study aimed to confirm whether serum miR-224 level is associated with the presence of venous metastasis and survival. Methods. TaqMan miRNA probe was used to perform qRT-PCR assays to evaluate the expression of serum miR-224 in a cohort of 182 HCC patients. Results. Patients with high miR-224 serum level showed poor survival compared to that with low miR-224 serum level (HR 1.985; 95% CI, 1.08, 3.65, ). The serum miR-224 levels were significantly higher in the BCLC stage C patients compared with the stage B patients (). In further analysis, significant difference of serum miR-224 expression level was observed when patients grouped by the status of PVTT but not the status of extra-liver metastasis ( and ). Serum levels of miR-224 showed significant relation with parameters of liver damage and serum AFP. Conclusion. Serum miR-224 might be BCLC stage dependent. It can reflect the status of tumor and liver damage. It was an independent predictor for the survival of HCC patients.