Advanced Glycation End Products Enhance Macrophages Polarization into M1 Phenotype through Activating RAGE/NF-κB Pathway

Figure 7

Effects of RAGE/NF-κB pathway blocking treatment on M1 markers expression induced by AGEs. The cells were cultured with AGEs or BSA 500 μg/mL for 24 h. For pathway blocking treatment, cells were pretreated with anti-RAGE antibody (50 μg/mL) or PDTC (100 μM) for 60 min before AGEs stimulation. The mRNA levels were assayed by real time PCR and protein expression was assayed by FCM. Pretreatment with anti-RAGE Ab or PDTC attenuated effects of AGEs on expression of iNOS and CD86 (a). Pretreatment with anti-RAGE Ab or PDTC attenuated effects of AGEs on the expression of mRNA of IL-6 and TNF-α (b). ; ; and compared with AGEs group. MFI: mean fluorescence intensity. Each bar represents the mean value ± SD of triplicate determinations, representative of three independent experiments.

(a)

(b)