Development of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Trichosporon asahii in Experimental and Clinical Samples

Figure 1

Specificity of LAMP detection for different T. asahii strains. Lane 1: negative control; lane 2: standard T. asahii CBS2479; lane 3: T. asahii CBS8904; lane 4: T. asahii CBS7137; lane 5: T. asahii BZ701; lane 6: T. asahii BZ702; lane 7: T. asahii BZ703; lane 8: T. asahii BZ704; lane 9: T. asahii BZ705; lane 10: T. asahii BZ706; lane 11: T. asahii BZ707; lane 12: T. asahii BZ708; lane 13: T. asahii BZ709; lane 14: T. asahii BZ710; lane 15: T. asahii BZ901; lane 16: T. asahii BZ902. (a) Specificity of LAMP assay detected by real-time measurement of turbidity (LA-320C). A positive reaction was defined as a threshold value of >0.1 within 80 min. A positive reaction was observed in all T. asahii isolates, whereas the negative controls showed no increase in turbidity. (b) Electrophoresis (1%) applied to loop-mediated amplification products from different T. asahii strains. The positive reaction was seen as a ladder-like pattern on 1% agarose gel electrophoresis analysis. (c) The specificity of LAMP for T. asahii detection by direct observation. A green colour was observed using the naked eye in the tube which contained T. asahii, whereas the negative controls remained light orange after the reaction.

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