Nordihydroguaiaretic Acid Disrupts the Antioxidant Ability of Helicobacter pylori through the Repression of SodB Activity In Vitro

Figure 2

Effect of NDGA on intracellular Fe²⁺ levels, SOD activity, and bacterial growth. (a) Intracellular Fe²⁺ levels in ATCC700392 exposed to NDGA and fecA1-deletion mutant of ATCC700392 (ΔfecA1) were measured by the method described under Materials and Methods. Results are means ± SD of three independent assays. ; (Tukey test). (b) The SodB activities in ATCC700392 exposed to NDGA and fecA1-deletion mutant of ATCC700392 (ΔfecA1) were measured by the method described under Materials and Methods. Results are means ± SD of three independent assays. ; (Tukey test). (c) The growth rates of ATCC700392 without NDGA (white circle), ATCC700392 exposed to 10 μM NDGA (gray circle), ATCC700392 exposed to 50 μM NDGA (deep-gray circle), ATCC700392 exposed to 100 μM NDGA (black circle), and fecA1-deletion mutant of ATCC700392 (white triangle) were measured by measuring optical density (OD 600 nm).

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