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BioMed Research International
Volume 2015, Article ID 737683, 9 pages
http://dx.doi.org/10.1155/2015/737683
Research Article

Fixation of Light Weight Polypropylene Mesh with n-Butyl-2-cyanocrylate in Pelvic Floor Surgery: Experimental Design Approach in Sheep for Effectiveness Evaluation

1Department of Cell Biology, Physiology and Immunology, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, 08153 Barcelona, Spain
2Integrated Services of Laboratory Animals, Universitat Autònoma de Barcelona, Bellaterra, 08153 Barcelona, Spain
3B. Braun Surgical S.A., Rubi, 08191 Barcelona, Spain
4Department of Animal Medicine and Surgery, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, 08153 Barcelona, Spain
5Department of Animal Health and Anatomy, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, 08153 Barcelona, Spain

Received 29 March 2015; Accepted 15 June 2015

Academic Editor: Andrea Tinelli

Copyright © 2015 Sandra Barbosa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. The aim of this study was to find a proper experimental design and to evaluate n-butyl-2-cyanoacrylate (Histoacryl) as a fixation method for a light-weight and large pore PP mesh (Synthetic PP Mesh-1) using the sheep as an animal model. Methods. Posterior vaginal implantation by means of episiotomy was used to implant 8 ewes which were evaluated macroscopically and histologically at 3 months () and 6 months () post-surgery. In previous pilot studies anterior vaginal implantation was evaluated, as well as different synthetic mesh materials, sizes and fixation methods ( to 3) during three weeks. In all cases a clinical evaluation of the animal was performed. Results. A reduction in the mesh size (Synthetic PP Mesh-1) together with precise application of the surgical glue Histoacryl to fix the mesh yielded significantly better histocompatibility results () compared to larger size or other fixation methods. Conclusion. The combination of Synthetic PP Mesh-1 with Histoacryl offered a high degree of graft integration without vaginal ulceration and a minimal foreign body reaction, being the sheep a proper animal model to test these types of medical devices.