The Effect of Walterinnesia aegyptia Venom Proteins on TCA Cycle Activity and Mitochondrial NAD⁺-Redox State in Cultured Human Fibroblasts

Table 1

The effect of incubation of confluent fibroblast cultures with 10 μg of Walterinnesia aegyptia venom proteins on the activities of TCA cycle enzymes and mitochondrial NADP⁺-dependent isocitrate dehydrogenase.

Samples (); Control/venom protein	Enzyme specific activities; nmole/min/mg protein or μmole/min/mg protein for MDH
	CS	ACN		α-KGDH	SDH	MDH	Mitochondrial
Control
Crude venom
F1
F2
F3
F4
F5
F6
F7

The values shown here are mean ± SD determined for mitochondrial sonicates of duplicate flasks of ten cultures. CS = citrate synthase, ACN = aconitase, = isocitrate dehydrogenase, α-KGDH = α-ketoglutarate dehydrogenase, SDH = succinate dehydrogenase, and MDH = malate dehydrogenase. = isocitrate dehydrogenase. ^*Significant at obtained upon comparison of the specific activities of all enzymes in mitochondrial sonicates of cells incubated with the crude venom and fractions F3–F7 against the respective activities recorded for controls. obtained upon comparison of the specific activities of all enzymes in mitochondrial sonicates of cells incubated with F3 and F6 against the respective activities recorded for cells incubated with F4, F6, and F7.