BioMed Research International

Research Article

The Effect of Walterinnesia aegyptia Venom Proteins on TCA Cycle Activity and Mitochondrial NAD+-Redox State in Cultured Human Fibroblasts

Table 2

The effect of incubation of mitochondrial fibroblast sonicate with 10 μg of Walterinnesia aegyptia venom proteins on the activities of TCA cycle enzymes and mitochondrial NADP+-dependent isocitrate dehydrogenase.
Sonicates and venom fractions
Assayed samples
()		 Enzyme specific activities
nmole/min/mg protein or μmole/min/mg protein for MDH
CSACNα-KGDHSDHMDHMitochondrial
Sonicate
Sonicate + crude venom
Sonicate + F1
Sonicate + F2
Sonicate + F3
Sonicate + F4
Sonicate + F5
Sonicate + F6
Sonicate + F7
Values shown here are mean ± SD determined for mitochondrial sonicates of duplicate flasks of ten cultures. CS = citrate synthase, ACN = aconitase, = isocitrate dehydrogenase, α-KGDH = α-ketoglutarate dehydrogenase, SDH = succinate dehydrogenase, MDH = malate dehydrogenase, and = isocitrate dehydrogenase. No statistically significant differences between all sets of data were observed.