Phytochemical Screening and Acute Oral Toxicity Study of Java Tea Leaf Extracts

Table 1

Retention times, MS, and MS/MS values of the major constituents present in OS leaves crude extracts identified via QTrap LCMS/MS with UHPLC system.

Peak	Retention time	Molecular ion peak (M-H)^−	MS^2 fragment ion intensity	Tentative compounds identified
1	2.1	117	73, 99.0, 69.7, 67.6, 71.5	Succinic acid
2	2.76	197	135.0, 123.0, 134.0, 179.0	Syringic acid
3	3.48	153	109, 65.0, 91.0, 108.0, 122.0	Protocatechuic acid
4	4.22	137	137, 108.0, 93.0, 65.0, 63.0	4-Hydroxy benzoic acid
5	4.46	174	135.0, 89.0, 108.0, 132.0	Caffeic acid
6	5.52	359.1	133, 123, 161.1, 179.1, 197.1	Rosmarinic acid
7	6.2	193.1	133.0, 137.0, 161.0	Ferulic acid
8	6.62	285	133, 65, 175.1, 199.0, 217.1	Luteolin
9	8.20	299.1	284.1, 133.0, 227.2, 256.2, 211.2	Kaempferol methyl ether
10	8.47	343.1	270.1, 170.3, 198.3, 214.3	Tetramethoxy chalcone derivative
11	9.27	316.3	315.3, 297.4, 197.2, 185.2, 169.2	Protocatechuic acid hexoside

Base peak.