Nucleofection of Rat Pheochromocytoma PC-12 Cells with Human Mutated Beta-Amyloid Precursor Protein Gene (APP-sw) Leads to Reduced Viability, Autophagy-Like Process, and Increased Expression and Secretion of Beta Amyloid

<div>Analysis of protein expression. Letter “M” indicates mock-nucleofected cells, while G, W, S stand for PC-12 cells nucleofected with GFP vector (G), or GFP vector + APP-wt (W), or GFP vector + APP-sw (S), respectively. (a) Identification of APP, sAPPα, clusterin, LC3-II, Aβ 1-16, and beta-actin in whole cell lysates isolated from six subsequent nucleofections; (b) identification of VPS34, Beclin 1, clusterin, LC3-II, and beta-actin in whole cell lysates isolated from cells nucleofected with GFP vector (G), or GFP vector + APP-wt (W), or GFP vector + APP-sw (S).</div>

BioMed Research International

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Figure 3: Nucleofection of Rat Pheochromocytoma PC-12 Cells with Human Mutated Beta-Amyloid Precursor Protein Gene (APP-sw) Leads to Reduced Viability, Autophagy-Like Process, and Increased Expression and Secretion of Beta Amyloid

Figure 3 | Nucleofection of Rat Pheochromocytoma PC-12 Cells with Human Mutated Beta-Amyloid Precursor Protein Gene (APP-sw) Leads to Reduced Viability, Autophagy-Like Process, and Increased Expression and Secretion of Beta Amyloid 