Activated Integrin-Linked Kinase Negatively Regulates Muscle Cell Enhancement Factor 2C in C2C12 Cells

Figure 1

Inhibition of PI3K inhibited ILK and MEF2C phosphorylation activity and the expression of MCK mRNA. (a), (b), and (c) Changes in ILK and MEF2C expression in LY294002-treated (L+/C−) C2C12 cells were not obvious; ILK (, ) and MEF2C (, ) phosphorylation levels decreased. The 3 h treatment with 20 μM LY294002 had a clearly inhibitory effect on ILK phosphorylation and MEF2C phosphorylation levels. (d) MCK mRNA levels in C2C12 cells treated with LY294002 (L+/C−) were significantly lower (, ) than that in the control (L−/C−).

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