Activated Integrin-Linked Kinase Negatively Regulates Muscle Cell Enhancement Factor 2C in C2C12 Cells

Figure 3

ILK-shRNA enhanced MEF2C phosphorylation activity and MCK mRNA expression. (a), (b) ILK-1048 was the most potent and selected for further study. (c), (d), and (e) Changes in MEF2C expression were not obvious with ILK-shRNA (ILK-1048) in C2C12 cells; ILK (, ) phosphorylation levels decreased significantly and MEF2C phosphorylation levels increased significantly (, ). (d) MCK mRNA levels in C2C12 cells treated with Cpd22 were significantly increased (, ) compared with that in the control.

(a)

(b)

(c)

(d)

(e)

(f)