Extracellular Calcium-Dependent Modulation of Endothelium Relaxation in Rat Mesenteric Small Artery: The Role of Potassium Signaling

Figure 4

The concentration-dependent relaxation to ACh in the bath solutions containing 4.2 mM ((a) ) and 5.9 mM ((b) ) . Responses were compared after preincubation with 100 μM L-NAME and 3 μM indomethacin (grey curves) and after preincubation with 100 μM L-NAME, 3 μM indomethacin, and 1 μM TRAM34 (black curves). (c) shows the areas under curve (AUC) for the concentration-response curves shown in (a) and (b). (d) shows a shift in sensitivities to ACh (pD₂) after addition of TRAM34 in the experiments shown in (a) and (b). (e) shows changes in the maximal relaxations (to 10 μM ACh) for the experiments shown in (a) and (b). +, ++, and +++ indicate , 0.01, and 0.001 for responses in the presence of 1 mM versus 2.5 mM . , , and indicate , 0.01, and 0.001 for responses before and after incubation with TRAM34.

(a)

(b)

(c)

(d)

(e)