Magnetic Resonance Imaging of Atherosclerosis Using CD81-Targeted Microparticles of Iron Oxide in Mice

Figure 6

Histological identification of MPIO. (a) Representative H&E staining of an aortic artery section from the mice receiving CD81-MPIO. (b) Enlargement of the area from (a) shows MPIO on the surface of the atherosclerotic plaque (arrows). (c) Immunohistochemical analysis CD81 expression in atherosclerotic plaque associated with the aortic root. 6 μm contiguous histological sections were taken in a similar orientation to the ex vivo MRI of the aortic root. Immunohistochemical staining was used to demonstrate CD81 expression (brown). (d) Enlargement of the area from (c) shows CD81-positive cells inside the atherosclerotic plaque. Scale bars are 100 μm for images (a) and (c), 20 μm for images (b) and (d).

(a)

(b)

(c)

(d)