Helicobacter pylori CagA Suppresses Apoptosis through Activation of AKT in a Nontransformed Epithelial Cell Model of Glandular Acini Formation

Figure 2

H. pylori does not induce proliferation of MCF-10A cell. MCF-10A cells were 3D cultured and infected with H. pylori strains. (a) Example of the average size of the acini. (b) The size of acini from three independent experiments was quantified at day 10, and no statistical differences were found. (c) 3D MCF-10A cells were stained with anti-Ki-67 antibody (green) and DAPI (blue) at different days of acini growth. (d) Confocal microscopy sections were made at 25, 50, and 75% deep of the acini. Red arrows indicate H. pylori (green) presence in close contact with the acini luminal cells (DAPI). (e) Confocal microscopy sections were made at 50% deep of the acini; image shows CagA (green) and nuclei (DAPI) in the acini central cells. (f) Monolayers of MCF-10A cells labeled with CFSE were infected with H. pylori strains for 3 days. No statistical differences were found in the rate of proliferation between the different infected cells.

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