Simultaneous Treatment with Statins and Aspirin Reduces the Risk of Prostate Cancer Detection and Tumorigenic Properties in Prostate Cancer Cell Lines

Figure 3

Effect of STA, ASA, and the combination of both treatments on prostate cancer cell lines invasion capacity. Values for invasion are presented as crystal violet absorbance/well for PC3 (a) and LNCaP (b) cell lines after 48 h of treatment with different conditions: nontreated cells, control (C) and control with DMSO (C(DMSO)), and cells treated with statin (STA), aspirin (ASA), and a combination of both statin and aspirin (STA + ASA) (for further details, see Section 2). The bars represent the means ± SEM. Values that are significantly different by one-way analysis of variance (ANOVA) from the control (C = nontreated) group are indicated by (versus C(DMSO)). Western blot of α2-integrin (150 KDa), vimentin (58 KDa), E-cadherin (120 KDa), and the control α-tubulin (52 KDa) was performed on total cell extracts after 7 days of treatment for PC3 cell line (c) and α2-integrin (150 KDa) and the control α-tubulin (52 KDa) for LNCaP (d). Densitometry analysis using Image J software is represented below bands as fold change values (versus C(DMSO)).

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