Regulation of Cell Cycle Regulators by SIRT1 Contributes to Resveratrol-Mediated Prevention of Pulmonary Arterial Hypertension

Figure 2

SIRT1 regulates expression of cell cycle regulatory molecules and arrests PDGF-BB-treated HPASMCs in G0/G1 phase. HPASMCs infected with adenoviral vectors encoding SIRT1 overexpression (a) or knockdown (b) were treated with PDGF-BB for indicated period. p21, cyclin D1, and cyclin E protein levels were analyzed by western blotting. Bar graphs show densitometric analysis of western blotting. The densitometric quantification was normalized to GAPDH. Data are shown as means ± SEM for three independent experiments. , , and versus 0 hour Ad-GFP group (a) or 0 hour Ad-U6 group (b); , versus corresponding Ad-GFP group (a) or Ad-U6 group (b) at the indicated time points. (c) Flow cytometry analysis of HPASMCs after SIRT1 overexpression and treatment with PDGF-BB (). versus Ad-GFP at the indicated time points.