Characterization of Bone Marrow Mononuclear Cells on Biomaterials for Bone Tissue Engineering In Vitro

Figure 2

Number and metabolic activity of adhering BMC over the time depending on surface coating and medium conditions. BMCs seeded on β-TCP were cultured over 21 days. (a) Number of adhering cells was determined at day 2 (white bars), day 7 (light grey bars), day 14 (dark grey bars), and day 21 (black bars) by means of DAPI staining and subsequent fluorescence microscopy as indicated in Materials and Methods section. Significantly highest numbers of adhering BMC were observed on β-TCP without coating and cultured in MesenCult medium (). without coating in DMEM and fibronectin coating in MesenCult, day 7 versus human plasma in MesenCult. without coating in MesenCult, day 2 versus human plasma in MesenCult. without coating in MesenCult, day 7, day 14, and day 21 versus all other groups. The changes of the metabolic activity over 21 days based on a function of surface coating and on medium conditions were presented in (b). The metabolic activity of BMC increased on all biomaterials in all experimental conditions but no statistically significant differences were found ().

(a)

(b)