Designation of a Novel DKK1 Multiepitope DNA Vaccine and Inhibition of Bone Loss in Collagen-Induced Arthritic Mice

Figure 4

The DNA vaccine attenuated bone erosion in CIA mice. Mice were injected intradermally with bovine type II collagen to induce arthritis (/group). (a-b) The incidence of arthritis and clinical score were recorded until day 39. (c–f) Representative joints on the day of sacrifice were analyzed with HE staining, Micro-CT images, BMD analysis, and TB staining images. Bars indicate 500 μm and 200 μm. Data are expressed as the mean ± SEM. .

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