Cloning and Expression of Synthetic Genes Encoding the Broad Antimicrobial Spectrum Bacteriocins SRCAM 602, OR-7, E-760, and L-1077, by Recombinant <i>Pichia pastoris</i>

<table class="table-group" id="tab5"><tr><td><table class="table"><tr><td class="thead-hr" colspan="4"><hr/></td></tr><tr class="thead"><td align="left">Bacteriocin</td><td align="center">Predicted molecular <br/>mass (Da)</td><td align="center">Trypsin-digested precursor (Da)</td><td align="left">Amino acid sequence <br/>(MS/MS analysis)</td></tr><tr><td class="thead-hr" colspan="4"><hr/></td></tr><tr><td align="left">SRCAM 602</td><td align="center">4,630.1</td><td align="center">926.65 <br/>1,500.95 <br/>1,614.97</td><td align="left">SANALRPPT  <br/>GDKENAAKASSVPAR  <br/>KTGGNRAVSGAGEIAAR</td></tr><tr><td align="left">OR-7</td><td align="center">6,215.1</td><td align="center">Deficient signal</td><td align="left">—</td></tr><tr><td align="left">E-760</td><td align="center">6,179.8</td><td align="center">1,166.63 <br/>1,579.68</td><td align="left">VGNPLHGIFGR <br/>SLSAYMFFANEQR</td></tr><tr><td align="left">L-1077</td><td align="center">4,002.6</td><td align="center">1,638.76 <br/>1,718.73</td><td align="left">IVGSQAGIGEYLFER <br/>LVELSEQELVDCER</td></tr><tr class="table-tr"><td colspan="4"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

Results obtained by MALDI-TOF/TOF MS analysis of the eluted RP-FPLC fractions from purified supernatants of the recombinant <i>P. pastoris </i>X-33 derivatives.

BioMed Research International

tab5

Table 5

Table 5: Cloning and Expression of Synthetic Genes Encoding the Broad Antimicrobial Spectrum Bacteriocins SRCAM 602, OR-7, E-760, and L-1077, by Recombinant <i>Pichia pastoris</i> 

Table 5 | Cloning and Expression of Synthetic Genes Encoding the Broad Antimicrobial Spectrum Bacteriocins SRCAM 602, OR-7, E-760, and L-1077, by Recombinant Pichia pastoris 