Research Article
Heterologous Reconstitution of Omega-3 Polyunsaturated Fatty Acids in Arabidopsis
Table 3
Fatty acids composition (% w/w) produced by transgenic Arabidopsis T2 lines expressing pCAM::D6ELD5 construct.
| | T2 group lines | Sample weight | 16:0 | 18:0 | 18:1 | LA | GLA | ALA | STA | 20:1 | DGLA | ARA | ETA | EPA | DTA | DPA |
| | WT | 0.1 | 8.5 ± 0.3 | 3.6 ± 0.2 | 15.1 ± 0.3 | 31.0 ± 0.3 | 0.0 ± 0.0 | 22.3 ± 0.1 | 0.0 ± 0.0 | 19.4 ± 0.3 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | | 1 | 0.1 | 8.6 ± 0.2 | 4.4 ± 0.1 | 17.1 ± 0.1 | 30.8 ± 0.1 | 0.6 ± 0.1 | 18.0 ± 0.1 | 1.3 ± 0.3 | 17.4 ± 0.3 | 0.7 ± 0.3 | 0.1 ± 0.0 | 1.0 ± 0.2 | 0.0 ± 0.0 | 0.0 ± 0.1 | 0.1 ± 0.0 | | 2 | 0.1 | 8.4 ± 0.3 | 4.3 ± 0.2 | 16.6 ± 0.3 | 30.2 ± 0.4 | 0.7 ± 0.2 | 19.1 ± 0.4 | 1.4 ± 0.1 | 17.2 ± 0.1 | 0.9 ± 0.1 | 0.1 ± 0.1 | 1.1 ± 0.4 | 0.0 ± 0.0 | 0.0 ± 0.1 | 0.1 ± 0.1 | | 5 | 0.1 | 8.8 ± 0.1 | 4.4 ± 0.3 | 15.6 ± 0.2 | 31.2 ± 0.1 | 0.7 ± 0.3 | 18.2 ± 0.4 | 1.5 ± 0.1 | 17.2 ± 0.1 | 1.0 ± 0.4 | 0.1 ± 0.1 | 1.2 ± 0.1 | 0.0 ± 0.0 | 0.0 ± 0.1 | 0.1 ± 0.0 | | 6 | 0.1 | 8.6 ± 0.4 | 4.0 ± 0.3 | 15.9 ± 0.5 | 31.4 ± 0.2 | 0.2 ± 0.1 | 19.4 ± 0.2 | 0.4 ± 0.4 | 19.8 ± 0.3 | 0.1 ± 0.1 | 0.0 ± 0.0 | 0.2 ± 0.1 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | | 11 | 0.1 | 9.5 ± 0.3 | 5.5 ± 0.1 | 14.1 ± 0.3 | 29.2 ± 0.1 | 1.6 ± 0.1 | 16.5 ± 0.3 | 3.6 ± 0.1 | 14.6 ± 0.4 | 2.3 ± 0.1 | 0.2 ± 0.1 | 2.5 ± 0.4 | 0.1 ± 0.0 | 0.0 ± 0.1 | 0.3 ± 0.0 | | 12 | 0.1 | 9.8 ± 0.5 | 6.1 ± 0.2 | 14.6 ± 0.3 | 28.6 ± 0.2 | 1.9 ± 0.3 | 14.8 ± 0.3 | 4.1 ± 0.4 | 13.5 ± 0.3 | 2.8 ± 0.2 | 0.2 ± 0.0 | 3.1 ± 0.4 | 0.2 ± 0.0 | 0.0 ± 0.0 | 0.3 ± 0.0 | | 14 | 0.1 | 9.2 ± 0.3 | 4.3 ± 0.4 | 14.7 ± 0.1 | 30.8 ± 0.1 | 1.0 ± 0.1 | 19.2 ± 0.1 | 2.4 ± 0.3 | 15.0 ± 0.1 | 1.4 ± 0.3 | 0.1 ± 0.1 | 1.9 ± 0.3 | 0.1 ± 0.0 | 0.0 ± 0.0 | 0.1 ± 0.0 | | 15 | 0.1 | 8.8 ± 0.2 | 4.8 ± 0.3 | 15.5 ± 0.1 | 30.4 ± 0.1 | 1.1 ± 0.4 | 17.2 ± 0.2 | 2.9 ± 0.2 | 15.3 ± 0.1 | 1.6 ± 0.2 | 0.2 ± 0.1 | 2.0 ± 0.5 | 0.1 ± 0.0 | 0.0 ± 0.0 | 0.2 ± 0.0 | | 18 | 0.1 | 10.1 ± 0.3 | 5.0 ± 0.2 | 12.3 ± 0.1 | 30.3 ± 0.2 | 1.8 ± 0.2 | 17.5 ± 0.4 | 3.6 ± 0.4 | 14.1 ± 0.1 | 2.2 ± 0.1 | 0.2 ± 0.2 | 2.4 ± 0.4 | 0.2 ± 0.0 | 0.0 ± 0.0 | 0.2 ± 0.0 | | 20 | 0.1 | 9.4 ± 0.4 | 4.7 ± 0.4 | 13.0 ± 0.3 | 30.9 ± 0.4 | 1.2 ± 0.1 | 18.3 ± 0.2 | 2.5 ± 0.1 | 16.6 ± 0.3 | 1.4 ± 0.3 | 0.1 ± 0.1 | 1.6 ± 0.1 | 0.1 ± 0.0 | 0.0 ± 0.0 | 0.2 ± 0.0 | | 21 | 0.1 | 8.8 ± 0.3 | 4.4 ± 0.1 | 16.6 ± 0.2 | 30.7 ± 0.4 | 1.1 ± 0.2 | 17.7 ± 0.4 | 2.2 ± 0.4 | 15.9 ± 0.4 | 1.0 ± 0.0 | 0.0 ± 0.0 | 1.4 ± 0.1 | 0.1 ± 0.0 | 0.0 ± 0.0 | 0.1 ± 0.0 | | 23 | 0.1 | 8.7 ± 0.1 | 4.3 ± 0.5 | 15.0 ± 0.5 | 30.9 ± 0.1 | 0.5 ± 0.4 | 19.3 ± 0.1 | 1.2 ± 0.1 | 18.4 ± 0.3 | 0.7 ± 0.2 | 0.1 ± 0.0 | 0.8 ± 0.3 | 0.1 ± 0.0 | 0.0 ± 0.0 | 0.1 ± 0.0 | | 24 | 0.1 | 8.8 ± 0.2 | 3.7 ± 0.4 | 14.2 ± 0.1 | 32.6 ± 0.3 | 0.1 ± 0.1 | 20.5 ± 0.1 | 0.2 ± 0.2 | 19.7 ± 0.1 | 0.1 ± 0.0 | 0.0 ± 0.0 | 0.1 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | | 26 | 0.1 | 8.7 ± 0.3 | 4.4 ± 0.3 | 15.4 ± 0.2 | 31.2 ± 0.2 | 0.6 ± 0.3 | 18.3 ± 0.4 | 0.9 ± 0.1 | 19.0 ± 0.2 | 0.8 ± 0.1 | 0.0 ± 0.0 | 0.7 ± 0.1 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.1 ± 0.0 | | 27 | 0.1 | 8.6 ± 0.4 | 4.2 ± 0.3 | 14.2 ± 0.3 | 31.7 ± 0.1 | 0.8 ± 0.1 | 18.4 ± 0.4 | 1.5 ± 0.3 | 17.8 ± 0.4 | 1.2 ± 0.3 | 0.1 ± 0.0 | 1.4 ± 0.4 | 0.1 ± 0.1 | 0.0 ± 0.0 | 0.1 ± 0.0 | | 28 | 0.1 | 8.7 ± 0.2 | 3.9 ± 0.2 | 14.5 ± 0.1 | 32.1 ± 0.1 | 0.2 ± 0.1 | 20.1 ± 0.2 | 0.4 ± 0.4 | 19.3 ± 0.4 | 0.3 ± 0.1 | 0.0 ± 0.0 | 0.4 ± 0.1 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | | 33 | 0.1 | 9.0 ± 0.3 | 5.0 ± 0.5 | 15.1 ± 0.2 | 30.2 ± 0.2 | 2.1 ± 0.1 | 13.7 ± 0.1 | 3.4 ± 0.1 | 14.7 ± 0.2 | 3.2 ± 0.4 | 0.2 ± 0.0 | 3.0 ± 0.1 | 0.2 ± 0.0 | 0.0 ± 0.0 | 0.3 ± 0.1 | | 39 | 0.1 | 8.5 ± 0.2 | 4.0 ± 0.3 | 15.9 ± 0.1 | 33.9 ± 0.1 | 0.1 ± 0.1 | 18.1 ± 0.2 | 0.2 ± 0.2 | 19.2 ± 0.1 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.1 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | | 41 | 0.1 | 9.1 ± 0.1 | 4.8 ± 0.2 | 13.4 ± 0.1 | 31.1 ± 0.3 | 1.5 ± 0.4 | 16.9 ± 0.3 | 2.8 ± 0.3 | 16.5 ± 0.4 | 1.7 ± 0.1 | 0.2 ± 0.1 | 1.8 ± 0.2 | 0.1 ± 0.1 | 0.0 ± 0.0 | 0.1 ± 0.0 | | 43 | 0.1 | 8.9 ± 0.4 | 5.4 ± 0.1 | 15.1 ± 0.1 | 29.2 ± 0.1 | 1.9 ± 0.2 | 16.7 ± 0.2 | 3.7 ± 0.3 | 15.5 ± 0.1 | 1.8 ± 0.3 | 0.0 ± 0.0 | 2.0 ± 0.1 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | | 44 | 0.1 | 9.1 ± 0.3 | 5.2 ± 0.3 | 13.2 ± 0.3 | 30.5 ± 0.2 | 1.5 ± 0.1 | 16.6 ± 0.3 | 2.6 ± 0.2 | 16.7 ± 0.1 | 2.1 ± 0.1 | 0.2 ± 0.0 | 2.0 ± 0.3 | 0.1 ± 0.0 | 0.1 ± 0.1 | 0.2 ± 0.1 | | 45 | 0.1 | 9.0 ± 0.2 | 4.4 ± 0.2 | 15.4 ± 0.2 | 31.1 ± 0.1 | 0.9 ± 0.3 | 17.7 ± 0.3 | 1.8 ± 0.1 | 17.4 ± 0.2 | 1.2 ± 0.3 | 0.0 ± 0.0 | 1.1 ± 0.2 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | | 57 | 0.1 | 9.2 ± 0.1 | 5.0 ± 0.3 | 13.6 ± 0.1 | 29.7 ± 0.1 | 1.1 ± 0.4 | 18.7 ± 0.4 | 2.6 ± 0.1 | 16.1 ± 0.4 | 1.7 ± 0.4 | 0.0 ± 0.0 | 2.2 ± 0.4 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | | 61 | 0.1 | 9.1 ± 0.3 | 5.0 ± 0.4 | 14.2 ± 0.3 | 31.7 ± 0.5 | 1.2 ± 0.3 | 16.7 ± 0.3 | 2.7 ± 0.1 | 15.6 ± 0.1 | 1.9 ± 0.3 | 0.0 ± 0.0 | 2.1 ± 0.2 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | | 62 | 0.1 | 11.2 ± 0.3 | 5.6 ± 0.3 | 0.0 ± 0.0 | 36.2 ± 0.1 | 1.9 ± 0.1 | 18.9 ± 0.3 | 4.3 ± 0.3 | 16.4 ± 0.3 | 2.6 ± 0.3 | 0.0 ± 0.0 | 2.9 ± 0.2 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | | 63 | 0.1 | 9.5 ± 0.2 | 4.8 ± 0.2 | 14.0 ± 0.3 | 30.6 ± 0.2 | 2.3 ± 0.3 | 14.4 ± 0.3 | 3.9 ± 0.1 | 13.6 ± 0.4 | 3.1 ± 0.2 | 0.3 ± 0.0 | 2.9 ± 0.1 | 0.2 ± 0.0 | 0.1 ± 0.0 | 0.3 ± 0.0 | | 64 | 0.1 | 9.3 ± 0.3 | 4.5 ± 0.3 | 14.3 ± 0.2 | 31.9 ± 0.1 | 2.1 ± 0.1 | 14.8 ± 0.1 | 3.0 ± 0.1 | 14.2 ± 0.4 | 2.8 ± 0.1 | 0.2 ± 0.1 | 2.4 ± 0.4 | 0.2 ± 0.0 | 0.1 ± 0.1 | 0.4 ± 0.0 | | 65 | 0.1 | 8.7 ± 0.4 | 4.3 ± 0.4 | 16.8 ± 0.2 | 31.9 ± 0.2 | 1.3 ± 0.3 | 15.9 ± 0.2 | 2.6 ± 0.1 | 15.2 ± 0.3 | 1.8 ± 0.1 | 0.1 ± 0.0 | 1.1 ± 0.3 | 0.1 ± 0.0 | 0.0 ± 0.0 | 0.2 ± 0.0 |
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Each value is the mean ± SD from three independent experiments. Total lipid is mg lipid g−1 dry cells.
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