Heterologous Reconstitution of Omega-3 Polyunsaturated Fatty Acids in Arabidopsis
Table 5
Fatty acids composition (% w/w) produced by transgenic Arabidopsis T3 lines expressing pCAM::D6ELD5 construct.
Usual FA
WT
33-1
33-2
33-4
44-1
44-4
44-5
63-1
63-5
64-3
Fatty acid
16:00
8.5 ± 0.3
8.8 ± 0.4
8.9 ± 0.1
8.7 ± 0.3
8.5 ± 0.2
8.6 ± 0.3
8.5 ± 0.1
9.4 ± 0.1
9.3 ± 0.3
9.4 ± 0.1
18:00
3.8 ± 0.1
4.2 ± 0.1
4.7 ± 0.1
4.8 ± 0.1
4.2 ± 0.2
4.2 ± 0.3
3.9 ± 0.4
5.3 ± 0.3
5.1 ± 0.2
5.1 ± 0.2
18:01
13.5 ± 0.1
12.6 ± 0.1
13.6 ± 0.1
13.3 ± 0.3
13.6 ± 0.1
13.4 ± 0.4
12.6 ± 0.3
13.5 ± 0.1
13.4 ± 0.3
13.4 ± 0.1
LA
29.6 ± 0.3
28.4 ± 0.1
27.5 ± 0.2
27.6 ± 0.4
30.5 ± 0.3
29.5 ± 0.2
29.8 ± 0.1
27.4 ± 0.3
28.0 ± 0.2
28.2 ± 0.4
ALA
22.9 ± 0.1
19.7 ± 0.4
19.6 ± 0.3
19.2 ± 0.1
20.9 ± 0.2
21.7 ± 0.3
22.9 ± 0.2
15.3 ± 0.1
17.5 ± 0.4
17.2 ± 0.3
20:01
21.7 ± 0.4
17.0 ± 0.1
16.8 ± 0.1
17.4 ± 0.1
20.9 ± 0.2
21.1 ± 0.2
21.6 ± 0.2
13.1 ± 0.3
17.0 ± 0.4
16.2 ± 0.1
Total
100
90.7
91.1
91
98.6
98.5
99.3
84
90.3
89.5
New -3 PUFA
STA
0.0 ± 0.0
3.3 ± 0.5
3.2 ± 0.4
3.1 ± 0.5
0.5 ± 0.1
0.6 ± 0.1
0.2 ± 0.0
5.6 ± 0.1 (26.8%, D6-des)
3.2 ± 0.1
3.5 ± 0.4
ETA
0.0 ± 0.0
2.2 ± 0.1
2.2 ± 0.1
2.2 ± 0.1
0.4 ± 0.1
0.4 ± 0.1
0.2 ± 0.0
3.8 ± 0.1 (40.4%, C18-elo)
2.3 ± 0.1
2.5 ± 0.3
EPA
0.0 ± 0.0
0.2 ± 0.2
0.2 ± 0.1
0.1 ± 0.0
0.0 ± 0.0
0.0 ± 0.0
0.0 ± 0.0
0.4 ± 0.0 (9.5%, D5-des)
0.2 ± 0.0
0.2 ± 0.0
DPA
0.0 ± 0.0
0.1 ± 0.0
0.1 ± 0.0
0.2 ± 0.0
0.0 ± 0.0
0.0 ± 0.0
0.0 ± 0.0
0.3 ± 0.0 (42.9%, C20-elo)
0.2 ± 0.0
0.2 ± 0.0
Total
0
5.8
5.7
5.6
0.9
1
0.4
10.1
5.9
6.4
New -6 PUFA
GLA
0.0 ± 0.0
1.3 ± 0.2
1.2 ± 0.1
1.3 ± 0.1
0.2 ± 0.1
0.2 ± 0.2
0.1 ± 0.1
2.3 ± 0.1 (7.7%, D6-des)
1.4 ± 0.3
1.7 ± 0.4
DGLA
0.0 ± 0.0
1.8 ± 0.4
1.7 ± 0.1
1.8 ± 0.2
0.3 ± 0.0
0.3 ± 0.0
0.2 ± 0.2
3.2 ± 0.5 (58.2%, C18-elo)
2.1 ± 0.4
2.2 ± 0.2
ARA
0.0 ± 0.0
0.3 ± 0.0
0.2 ± 0.0
0.2 ± 0.0
0.0 ± 0.0
0.0 ± 0.0
0.0 ± 0.0
0.3 ± 0.0 (8.6%, D5-dea)
0.2 ± 0.0
0.1 ± 0.0
DTA
0.0 ± 0.0
0.1 ± 0.0
0.1 ± 0.0
0.1 ± 0.0
0.0 ± 0.0
0.0 ± 0.0
0.0 ± 0.0
0.1 ± 0.0 (25.0%, C20-elo)
0.1 ± 0.0
0.1 ± 0.0
Total
0
3.5
3.2
3.4
0.5
0.5
0.3
5.9
3.8
4.1
Total new FA
0
9.3
8.9
9
1.4
1.5
0.7
16
9.7
10.5
Total FA
100
100
100
100
100
100
100
100
100
100
Each value is the mean ± SD from five independent experiments. Total lipid is mg lipid g−1 dry cells. Where shown, conversion efficiencies are calculated as [product]/[product + substrate] × 100. This value represents the overall percent of conversion of total substrate into total product formed.
