Effect of Metformin on Viability, Morphology, and Ultrastructure of Mouse Bone Marrow-Derived Multipotent Mesenchymal Stromal Cells and Balb/3T3 Embryonic Fibroblast Cell Line

Figure 3

Results of BrdU incorporation assay. (a) Cytotoxic effect of 10 mM metformin was recorded starting from 24th hour of BMSC culture. Metformin at 5 mM concentration significantly reduced DNA synthesis in BMSC cultures after 72 hours of treatment. Metformin at the lowest investigated concentration did not affect DNA synthesis. (b) Increase of DNA synthesis was observed in Balb/3T3 after 48 hours of culture with 1 mM metformin. Cytotoxic effect of metformin was prominent after 72 hours of culture, especially when Balb/3T3 were treated with metformin at 5 and 10 mM doses. Fold change in DNA synthesis was calculated by comparing BrdU signals of metformin-treated cells to that of the control culture, to which a value of 1 was assigned. An asterisk () indicates a statistically significant difference (). Significance was determined by two-way ANOVA test ().

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