Effect of Metformin on Viability, Morphology, and Ultrastructure of Mouse Bone Marrow-Derived Multipotent Mesenchymal Stromal Cells and Balb/3T3 Embryonic Fibroblast Cell Line

Table 1

Sequences of primers used in qPCR.

Gene	Abbreviation	Sequence 5′-3′	Loci	Amplicon length [bp]	Accession number
Beta-2 microglobulin	b2m	F: CATACGCCTGCAGAGTTAAGCA	341–362	73	NM_009735.3
		R: GATCACATGTCTCGATCCCAGTAG	413–390
Insulin-like growth factor 2	IGF2	F: TCAGTTTGTCTGTTCGGACCG	223–243	223	NM_001122737.1
		R: TTGGAAGAACTTGCCCACG	445–427
Insulin-like growth factor 2 receptor	IGF2R	F: GGCTGCGATCGATATGCATCT	2616–2636	106	NM_010515.2
		R: GGCCTATCTTTGCAACTCCCA	2721–2701
H19	H19	F: AGGTGAAGCTGAAAG	2031–2045	97	NR_001592.1
		R: GCAGAGTTGGCCATGAAGATG	2127–2107
Octamer binding transcription factor 4	Oct-4	F: TTCTGCGGAGGGATGGCATA	258–277	232	NT_039649.8
		R: GTTCTAGCTCCTTCTGCAGGG	489–469