Paricalcitol Inhibits Aldosterone-Induced Proinflammatory Factors by Modulating Epidermal Growth Factor Receptor Pathway in Cultured Tubular Epithelial Cells

Figure 4

Aldosterone transactivates EGFR via TGFα, but not HB-EGF, shedding. Cells were pretreated for 24 h with a neutralizing antibody against TGFα (2.5 μg/mL) (a) or 1 hour with the HB-EGF pharmacological inhibitor CRM197 (10 μmol/L) (b) before stimulation with 1 μmol/L Aldo for 15 min. Western blot experiment and data are expressed as mean ± SEM of 3 or 5 independent experiments, respectively. versus control. ^# versus Aldo.

(a)

(b)