Influence of Culture Media on Biofilm Formation by Candida Species and Response of Sessile Cells to Antifungals and Oxidative Stress

Figure 1

Biofilm formation by C. albicans, C. glabrata, C. krusei, and C. parapsilosis. Yeast cells grown as described in Materials and Methods were harvested by low-speed centrifugation and suspensions were adjusted to a final density of 1.0 in 100 μL of either YP or YNB media supplemented or not, with 0.2 or 2% glucose. Cells were placed in wells of flat-bottomed 96-well microtiter plates (Nunc, Nalgene) and incubated at 37°C. After 12 h (a), 24 h (b), and 48 h (c), biofilm formation was assessed and planktonic cells were removed by washing the wells with sterile 10 mM calcium chloride. Plates were photographed with a Syngene Gene Genius Bio Imaging system.

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(b)

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