Influence of Culture Media on Biofilm Formation by Candida Species and Response of Sessile Cells to Antifungals and Oxidative Stress

Table 1

Susceptibility of planktonic and sessile cells to antifungals and AgNPs. Planktonic (either from logarithmic phase or stationary phase) and sessile cells of the four Candida species were diluted to an 0.1 with sterile deionized water.

(a) Aliquots of dilutions were incubated at 37°C in the presence of increasing concentrations of the indicated antifungals

Species	Ketoconazole (µg/mL)						Ketoconazole (µg/mL)  + AgNPs			Miconazole (µg/mL)						Miconazole (µg/mL); + AgNPs
	LP		SP		B		LP	SP	B	LP		SP		B		LP	SP	B
	S	MH	S	MH	S	MH	MH	MH	MH	S	MH	S	MH	S	MH	MH	MH	MH
C. albicans	256	2.1	2.1	2.1	256	256	6.9	0.05	0.05	6.9	6.9	6.9	256	256	6.9	0.05	0.05	0.05
C. glabrata	256	256	256	76.8	256	256	6.9	76.8	256	6.9	6.9	6.9	6.9	6.9	6.9	0.05	0.05	2.1
C. krusei	256	256	256	23	256	256	0.05	6.9	0.05	6.9	6.9	6.9	23	23	6.9	0.05	0.05	0.05
C. parapsilosis	76.8	6.9	6.9	76.8	6.9	6.9	6.9	0.05	6.9	76.8	23	23	76.8	76.8	76.8	0.05	0.05	6.9

(b) antifungals plus AgNPs. After 90 min, cells were pelleted by low-speed centrifugation and resuspended in sterile deionized water to an 0.1

Species	Fluconazole (µg/mL)						Fluconazole (µg/mL); + AgNPs			Itraconazole (µg/mL)						Itraconazole (µg/mL); + AgNPs
	LP		SP		B		LP	SP	B	LP		SP		B		LP	SP	B
	S	MH	S	MH	S	MH	MH	MH	MH	S	MH	S	MH	S	MH	MH	MH	MH
C. albicans	23	6.9	256	6.9	256	256	0.05	0.05	0.05	2.1	2.1	6.9	2.1	256	256	0.05	0.05	6.9
C. glabrata	256	256	256	76.8	256	256	0.05	256	256	76.8	6.9	256	6.9	256	256	0.05	0.05	76.8
C. krusei	256	256	256	256	256	256	0.05	0.05	0.05	76.8	2.1	256	6.9	256	256	0.05	0.05	0.05
C. parapsilosis	256	76.8	256	256	256	256	6.9	256	256	2.1	2.1	6.9	2.9	6.9	6.9	0.05	23	2.1

LP, logarithmic phase; SP, stationary phase; B, biofilm; AgNPs, silver nanoparticles; S, Sabouraud medium; MH, Mueller-Hinton medium.