Influence of Culture Media on Biofilm Formation by Candida Species and Response of Sessile Cells to Antifungals and Oxidative Stress

Table 2

Resistance of planktonic and sessile cells of Candida species to antifungals. Cultures in stationary phase of the four Candida species were diluted to an 0.1 with sterile deionized water. Aliquots of dilutions were incubated at 37°C in the presence of the indicated concentrations of antifungals. After 90 min, water was removed by low-speed centrifugation and the corresponding cell pellets were resuspended in sterile deionized water to an 0.1. Samples of these suspensions were exponentially diluted in 96-well plates and each dilution was spotted onto Mueller-Hinton agar plates that were incubated at 37°C. Growth was inspected after 48 h. LP, logarithmic phase; SP, stationary phase; B, biofilm.

Species	Fluconazole (µg/mL)			Itraconazole (µg/mL)			Ketoconazole (µg/mL)			Miconazole (µg/mL)
	LP	SP	B	LP	SP	B	LP	SP	B	LP	SP	B
C. albicans	256	256	256	256	256	256	6.9	256	256	23	256	76.8
C. glabrata	256	256	256	256	256	256	23	76.8	256	6.9	76.8	256
C. krusei	256	256	256	256	256	256	23	256	256	76.8	256	256
C. parapsilosis	256	256	256	256	256	256	76.80	256	256	23	256	256