Optimizing Perfusion-Decellularization Methods of Porcine Livers for Clinical-Scale Whole-Organ Bioengineering

Figure 2

Whole-organ porcine liver decellularization using different detergents. Representative images of (a) hematoxylin and eosin staining and (b) DAPI staining. Scale bars: (a, b) 100 μm. Normal liver was included as a control. (c) The DNA content of the decellularized liver matrix. versus the normal liver group. (d) The content of residual SDS in the decellularized liver matrix of three groups (SDS, S-SDS, and T-SDS) before and after the SDS elimination step via Triton (N.D.: not detected) ( for each group).

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