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BioMed Research International
Volume 2015, Article ID 790170, 10 pages
http://dx.doi.org/10.1155/2015/790170
Research Article

Guidelines for Optimisation of a Multiplex Oligonucleotide Ligation-PCR for Characterisation of Microbial Pathogens in a Microsphere Suspension Array

1Department of Microbial and Molecular Systems, KU Leuven, Kasteelpark Arenberg 20, Bus 2460, 3001 Leuven, Belgium
2Platform Biotechnology and Molecular Biology, Scientific Institute of Public Health (WIV-ISP), Juliette Wytsmanstraat 14, 1050 Brussels, Belgium
3National Reference Centre for Salmonella and Shigella, Bacterial Diseases Division, Communicable and Infectious Diseases, Scientific Institute of Public Health (WIV-ISP), Juliette Wytsmanstraat 14, 1050 Brussels, Belgium
4Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, 9052 Ghent, Belgium
5Department of Information Technology, Ghent University, IMinds, Gaston Crommenlaan 8, 9050 Ghent, Belgium

Received 16 September 2014; Revised 5 January 2015; Accepted 12 January 2015

Academic Editor: Marco Fichera

Copyright © 2015 Véronique Wuyts et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Citations to this Article [11 citations]

The following is the list of published articles that have cited the current article.

  • Véronique Wuyts, Wesley Mattheus, Nancy H. C. Roosens, Kathleen Marchal, Sophie Bertrand, and Sigrid C. J. De Keersmaecker, “A multiplex oligonucleotide ligation-PCR as a complementary tool for subtyping of Salmonella Typhimurium,” Applied Microbiology and Biotechnology, 2015. View at Publisher · View at Google Scholar
  • Pieter-Jan Ceyssens, Cristina Garcia-Graells, Frédéric Fux, Nadine Botteldoorn, Wesley Mattheus, Véronique Wuyts, Sigrid De Keersmaecker, Katelijne Dierick, and Sophie Bertrand, “ Development of a Luminex xTAG ® assay for cost-effective multiplex detection of β-lactamases in Gram-negative bacteria ,” Journal of Antimicrobial Chemotherapy, vol. 71, no. 9, pp. 2479–2483, 2016. View at Publisher · View at Google Scholar
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  • Xiaoqiang Wang, Sisi Ying, Xiaoguang Wei, and Jun Yuan, “Development of a gold nanoparticle-based universal oligonucleotide microarray for multiplex and low-cost detection of foodborne pathogens,” International Journal of Food Microbiology, vol. 253, pp. 66–74, 2017. View at Publisher · View at Google Scholar
  • V?ronique Wuyts, Wesley Mattheus, Nancy H. C. Roosens, Kathleen Marchal, Sophie Bertrand, and Sigrid C. J. De Keersmaecker, “Molecular Subtyping of Salmonella Typhimurium with Multiplex Oligonucleotide Ligation-PCR (MOL-PCR),” Diagnostic Bacteriology, vol. 1616, pp. 39–69, 2017. View at Publisher · View at Google Scholar
  • Xavier Libert, Ann Packeu, Nancy H. Roosens, Fabrice Bureau, and Sigrid C. J. De Keersmaecker, “Development and performance assessment of a luminex xMAP® direct hybridization assay for the detection and identification of indoor air fungal contamination,” PLoS ONE, vol. 12, no. 3, 2017. View at Publisher · View at Google Scholar
  • Nikol Reslova, Veronika Michna, Martin Kasny, Pavel Mikel, and Petr Kralik, “xMAP Technology: Applications in Detection of Pathogens,” Frontiers in Microbiology, vol. 8, 2017. View at Publisher · View at Google Scholar
  • Pengyu Zhu, Wei Fu, Shuang Wei, Xiao Liu, Chenguang Wang, Yun Lu, Ying Shang, Xiyang Wu, Yuping Wu, and Shuifang Zhu, “A high-throughput and ultrasensitive identification methodology for unauthorized GMP component based on suspension array and logical calculator,” Scientific Reports, vol. 9, no. 1, 2019. View at Publisher · View at Google Scholar
  • Eleonora Ventola, Bert Bogaerts, Sigrid C. J. De Keersmaecker, Kevin Vanneste, Nancy H. C. Roosens, Wesley Mattheus, and Pieter‐Jan Ceyssens, “Shifting national surveillance of Shigella infections toward geno‐serotyping by the development of a tailored Luminex assay and NGS workflow,” MicrobiologyOpen, pp. e00807, 2019. View at Publisher · View at Google Scholar
  • Nikol Reslova, Veronika Huvarova, Jakub Hrdy, Martin Kasny, and Petr Kralik, “A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay,” Scientific Reports, vol. 9, no. 1, 2019. View at Publisher · View at Google Scholar
  • Mathieu Gand, Wesley Mattheus, Nancy H.C. Roosens, Katelijne Dierick, Kathleen Marchal, Sigrid C.J. De Keersmaecker, and Sophie Bertrand, “A multiplex oligonucleotide ligation-PCR method for the genoserotyping of common Salmonella using a liquid bead suspension assay,” Food Microbiology, pp. 103394, 2019. View at Publisher · View at Google Scholar