Guidelines for Optimisation of a Multiplex Oligonucleotide Ligation-PCR for Characterisation of Microbial Pathogens in a Microsphere Suspension Array

Figure 4

Boxplot of signal-to-noise ratios for different combinations of amount of microspheres in the hybridisation reaction and streptavidin-R-phycoerythrin (SAPE) concentration in the reporter mix. Results of one of 2 independent tests are presented, that is, results of 6 isolates × 6 conditions = 36 MOL-PCR assays (for each of the 6 conditions: with and . 2500: 2500 microspheres of each region per reaction were combined in the microsphere mix; 375: 375 microspheres of each region per reaction were combined in the microsphere mix; 750: 750 microspheres of each region per reaction were combined in the microsphere mix; 4 µg: the reporter mix contained 4 μg/mL of SAPE; 10 µg: the reporter mix contained 10 μg/mL of SAPE; neg: expected negative results; pos: expected positive results; R: reference condition; : statistically significant difference with the reference condition.