Research Article

Cyclic Tensile Strain Induces Tenogenic Differentiation of Tendon-Derived Stem Cells in Bioreactor Culture

Table 1

Real-time PCR primers used in this study. Primers for type I collagen, tenascin-C, tenomodulin, and scleraxis and GAPDH were designed and synthesized by Sangon Biotech Co., Ltd.

Gene Sequence

Type I collagenForward 5′ tcagaacatcacctaccactgc 3′
Reverse5′ attgtctttccccattcatttg 3′

Tenascin-CForward5′ atcaccaccaagttcacaacag 3′
Reverse5′ ccatccacagattcatagagca 3′

TenomodulinForward5′ tccacaattcggcataatc 3′
Reverse5′ caggtccgggattctgtgt 3′

ScleraxisForward5′ ccacaccaagcattttcaga 3′
Reverse5′acacaaaggacggcatcac 3′

GAPDHForward5′ atggtgaaggtcggagtgaa 3′
Reverse5′ tgggtggaatcatactggaac 3′