Computational Analysis of the Binding Specificities of PH Domains

Table 1

Binding specificities of representative PH domains.

	Group 1	Group 2	Group 3	Group 4
PI(3,4,5)P3/Ins(1,3,4,5)P4	+	+	+	−
PI(4,5)P2/Ins(1,4,5)P3	−	+	−	−
PI(3,4)P2/Ins(1,3,4)P3	−	−	+	−
PH domain	Btk, Grp1, Gap1; Gap1, Vav, cytohesin-1, Sos, ARNO, TIAM1-N	PLC1, Ark, -spectrin, DAGK, RasGAp, OSBP, IRS-1, Plec-N	Dapp1, Akt, PDK1	Dynamin, TIAM1-C

+, − represent specific binding or nonspecific binding. Gap1 represents one of the members of Ras GTPase-activating proteins and Gap1 represents the mammalian counterpart of the Drosophila Gap1 gene.