[Retracted] N-(1-Pyrenyl) Maleimide Induces Bak Oligomerization and Mitochondrial Dysfunction in Jurkat Cells

Figure 1

NPM-induced apoptosis in Jurkat cells is dependent on caspase activation. (a) To determine the onset of caspase activation, Jurkat cells were treated with 0.5 μM NPM for the indicated time. The cell lysates were analyzed for the cleavage of caspases 3, 7, and PARP by Western blot. Actin served as a loading control. Data shown were from a representative of three independent experiments that gave similar results. (b) Effect of caspase inhibition on the viability of NPM-treated cells. Jurkat cells were pretreated with the broad-spectrum caspase inhibitor z-VAD-fmk (50 μM) for 1 h before exposing to 0.5 μM of NPM for 24 h. Cell viability was then determined by MTS assay and trypan blue exclusion method as described in Section 2. Data shown for MTS assay are the means ± SD from four independent experiments. Data shown for trypan blue exclusion method are the means ± SD from three independent experiments.

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