[Retracted] N-(1-Pyrenyl) Maleimide Induces Bak Oligomerization and Mitochondrial Dysfunction in Jurkat Cells

Figure 2

Effect of NPM on mitochondrial dysfunction in Jurkat cells. (a) Effect of NPM on the loss of mitochondrial membrane potential (). Jurkat cells were treated with 50 μM of CCCP (mitochondrial membrane potential disrupter) or 0.5–2 μM of NPM for 3 h. The treated cells were stained with JC-1 (5,5^′,6,6^′-tetrachloro-1,1^′,3,3^′ tetraethylbenzimidazolylcarbocyanine iodide) before being analyzed for the loss of mitochondrial membrane potential () by flow cytometry as described in Section 2. FL1-H: JC-1 green fluorescence. FL2-H: JC-1 red fluorescence. R1 and R2 are the percentage of cells with polarized and depolarized , respectively. (b) Effect of NPM on the release of cytochrome C from mitochondria to cytosol. Jurkat cells were treated with 0.5–2 μM of NPM or DMSO as control for 3 h. The treated cells were labeled with Anti-Cytochrome C-FITC antibody and analyzed by flow cytometry. FL1-H: cytochrome C-FITC. M1 and M2 are the percentage of cells with low (mitochondria that have released cytochrome C) and high (intact mitochondria) fluorescence, respectively. The M1 and M2 for the untreated cells (DMSO) are 6.6% and 93.4%, respectively. Data were from a representative of three independent experiments that gave similar results.

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