Identification of Human Herpesvirus 8 Sequences in Conjunctiva Intraepithelial Neoplasia and Squamous Cell Carcinoma of Ugandan Patients

Table 1

PCR primer sequences used to amplify HHV8 ORF26 regions by standard PCR and real time PCR.

Method	Locus	Primer name	Sequences (5′-3′)	Nucleotide position	Size	Reference
PCR outer	ORF26-3′	LGH2575-R	GTGCTTGACGATCTGTCC	47,638–47,655	620 bp	[30]
		LGH2574-L	CAGAAACAGGGCTAGGTAC	48,239–48,257
PCR inner	ORF26-3′	SJ-F	CTATCTTCAGAGTCTCAG	47,844–47,861	402 bp	[31]
		SJ-R	TAGGTACACACAATTTTG	48,228–48,245
PCR outer	ORF26-5′	LGH1701R	GGATCCCTCTGACAACC	47,292–47,309
		SJ-R2	GCCAAGATTAAATATAGAACTGAG	47,857–47,880	589 bp	[31]
PCR inner	ORF26-5′	LGH1701R	GGATCCCTCTGACAACC	47,292–47,309
		SJ-R1	AATATAGAACTGAGACTCTGAAG	47,848–47,870	579 bp	[31]
Real time PCR	ORF26-3′	ORF26LR1F1	GCAGTATCTATCCAAGTG	47,220–47,237
		ORF26LR2R2	ACAGATCGTCAAGCA	47,639–47,653	434 bp	This study

Nucleotide positions are given on the GenBank sequence number NC_009333.