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BioMed Research International
Volume 2015 (2015), Article ID 802697, 5 pages
http://dx.doi.org/10.1155/2015/802697
Research Article

Surgical Modification of the Murine Calvaria Osteolysis Model

1Department of Joint Surgery and Sports Medicine, The First Hospital of China Medical University, 155 Nanjing North Street, Shenyang, Liaoning 110001, China
2Key Laboratory of Diagnostic Radiology and Interventional Treatment, The First Hospital of China Medical University, 155 Nanjing North Street, Shenyang, Liaoning 110001, China

Received 27 July 2015; Revised 30 September 2015; Accepted 7 October 2015

Academic Editor: Carla R. Arciola

Copyright © 2015 Ali Mohammed Al-quhali et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The murine calvaria model has been adopted for evaluation of osteolysis and inflammation induced by polyethylene (PE) or metal wear debris. However, this model suffers from several complications. The purpose of our study is to introduce a surgical modification with lower complication rates, thus providing more accurate results. Forty C57/BL6 mice were divided into two groups, both receiving polyethylene particles. Surgical modifications were performed in group 1, and group 2 underwent traditional surgeries. The incidence of fluid leakage was recorded on the operative day. Curst formation, wound dehiscence, and bone exposure were recorded on day 7. Histological osteolysis was demonstrated by HE staining of tissue slices. Micro-CT was used for quantifying evaluation of osteolysis in two groups. Intraoperative fluid leakage was significantly reduced in group 1. Postoperative crust formation, wound dehiscence, and bone exposure were also significantly decreased in group 1. HE staining results revealed obvious osteolysis in group 1 and more obvious osteolysis in group 2. Bone volume fraction (BVF) was (0.32 ± 0.03) in group 1 compared to group 2 (0.24 ± 0.05). Bone mineral density (BMD) was (1.11 ± 0.03) in group 1 compared to group 2 (1.01 ± 0.02). Surgical modifications provide a reliable way for establishment of the murine calvaria osteolysis model.