Glutamine May Repress the Weak LPS and Enhance the Strong Heat Shock Induction of Monocyte and Lymphocyte HSP72 Proteins but May Not Modulate the HSP72 mRNA in Patients with Sepsis or Trauma

Figure 4

In healthy controls only LPS pretreated with Gln suppressed mHSP72 baseline expression (a). Gln given 1 hour before HS further enhanced the induced mHSP72 expression in comparison to Gln given 1 hour after induction (b). In SIRS Gln given 1 hour before LPS further suppressed the already LPS-suppressed mHSP72 baseline expression (c); in the HS induction model, Gln given before HS showed a trend to increase further the already induced lHSP72 expression (d). In septic PBMCs, only Gln given before LPS abolished the LPS induction effect on lHSP72 (e). However, Gln also given before and not after HS further induced lHSP72 baseline expression (f). These differences between HS and LPS and between Gln given before or after induction clearly underline a Gln pretreatment depressive trend to lymphocyte and monocyte HSP72 proteins of PBMCs exposed to LPS but an enhancing trend to those exposed to HS induction effect.

(a)

(b)

(c)

(d)

(e)

(f)