The Impact of Vitamin D₃ Supplementation on Mechanisms of Cell Calcium Signaling in Chronic Kidney Disease

Figure 3

The effect of vitamin D₃ supplementation on an inhibitory effect of AZ11645373. (a) The comparison of the AZ11645373 (50 nmol/L) effect on [Ca²⁺]_i in CKD patients at baseline and after vitamin D₃ supplementation (, , ). After vitamin D₃ supplementation, AZ11645373 (50 nmol/L) had no effect on [Ca²⁺]_i. (b) Differences in [Ca²⁺]_i after an inhibition with AZ11645373 (50 nmol/L) at baseline and after vitamin D₃ supplementation (, ). (c) The representative trace where AZ11645373 (50 nmol/L) inhibited the [Ca²⁺]_i rise induced by BzATP (50 μmol/L). (d) The comparison of the AZ11645373 (50 nmol/L) effect on BzATP- (50 μmol/L) activated calcium influx before and after supplementation (, ). (e) Differences in [Ca²⁺]_i in BzATP- (50 μmol/L) activated PBMCs after the AZ11645373 (50 nmol/L) inhibition at baseline and after vitamin D₃ supplementation (, ).

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