Vitamin D Receptor Gene Polymorphisms and Haplotypes in Hungarian Patients with Idiopathic Inflammatory Myopathy

Table 1

Sequences of specific primers and the profile of PCR used to amplify the DNA fragment carrying FokI, ApaI, and TaqI SNPs [28, 29].

	FokI polymorphism (rs2228570)	ApaI and TaqI polymorphisms (rs7975232 and rs731236)
Primers	5^′-AGC TGG CCC TGG CAC TGA CTC TGC TCT-3^′	5^′-CAG AGC ATG GAC AGG GAG CAA G-3^′
	5^′-ATG GAA ACA CCT TGC TTC TTC TCC CTC-3^′	5^′-GCA ACT CCT CAT GGC TGA GGT CTC A-3^′
PCR conditions (40 cycles)	30 sec at 95^°C	30 sec at 94^°C
	30 sec at 70^°C	1 min at 70^°C
	30 sec at 72^°C	1 min at 72^°C
Length of PCR product (bp)	265	740