Cloning and Characterization of Spliced Variants of the Porcine G Protein Coupled Receptor 120
Tissue and cell expression of total pGPR120. Primer GPR120-407 was designed for the common sequence of three variants which could produce the 407 bp fragment (a). The blue domain represents the common sequence, while the red domain represents the missed sequence of V2 and the green domain represents the missed sequence of V3. PCR products of total pGPR120 and β-actin were detected by 2% agarose gel electrophoresis (b). Lane 1: adipose; Lane 2: skeletal muscle; Lane 3: ileum; Lane 4: jejunum; Lane 5: duodenum; Lane 6: kidney; Lane 7: lung; Lane 8: spleen; Lane 9: liver; Lane 10: heart; M: DL2000 DNA marker. Samples were extracted from the cell lines, 3D4/2, PK15, and IPEC-J2, and the primary cells isolated from pigs, ADSC and DFAT. The expression of total pGPR120 was determined by q-PCR (c).