Cloning and Characterization of Spliced Variants of the Porcine G Protein Coupled Receptor 120

Figure 7

Effect of fatty acids on phosphorylation level of ERK1/2 in HEK293T cells transfected with pGPR120 expression plasmid or empty plasmid. HEK293T cells were transfected with pGPR120-WT expression plasmid or empty plasmid for 24 h. The cells were changed to the medium without FBS and maintained for at least 18 h. After a 10 min incubation of TUG-891 (10 μM), ALA (10 μM), EPA (10 μM), DHA (10 μM), LA (10 μM), or PLA (10 μM), cells were harvested to measure the phosphorylation level of ERK1/2.