Research Article
Cloning and Characterization of Spliced Variants of the Porcine G Protein Coupled Receptor 120
Table 1
Primers used for polymerase chain reaction.
| | Primer identification | Primer sequences (5′-3′) | Product size for WT (bp) | Product size for WT (bp) | Product size for WT (bp) | Annealing temperature (°C) |
| | GPR120 full | F: GAATTCGCCACCATGGGAATGTCCCTTGAGTGC
R: TCTAGACTAGCTGGAAATAACAGACAGATC | 1086 | 930 | 960 | 62 |
| | GPR120-407 | F: AAGGAGGAGGCTCACGATG
R: TGACAAATAGATGCCGATAGAC | 407 | 407 | 407 | 59 |
| | GPR120-Variant | F: GCTCTTCTACGTGATGACCCTA
R: CGTGAGCCTCCTCCTTGAT | 388 | 232 | 262 | 60 |
| | β-actin | F: CCAGGTCATCACCATCGG
R: CCGTGTTGGCGTAGAGGT | 158 | — | — | 60 |
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