Trichostatin A-Mediated Epigenetic Transformation of Adult Bone Marrow-Derived Mesenchymal Stem Cells Biases the In Vitro Developmental Capability, Quality, and Pluripotency Extent of Porcine Cloned Embryos

Table 2

Effect of the TSA-dependent epigenomic modulation of MSCs on the in vitro developmental outcome of cloned pig embryos.

TSA-mediated epigenetic transformation of MSCs	Number of oocytes/embryos			Development to
	Enucleated	Electrofused (%)	Cleaved (%)	Morulae (%)	Blastocysts (%)
+	186	178/186 (95.7)	174/178 (97.8)	155/178 (87.1)^a	116/178 (65.2)^A
−	293	275/293 (93.9)	262/275 (95.3)	216/275 (78.5)^b	125/275 (45.5)^B

TSA: trichostatin A; MSC: mesenchymal stem cell.
Values with different small superscript letters (a and b) within the same column denote statistically significant differences between experimental groups ( < 0.05; test). Values with different large superscript letters (A and B) within the same column denote very highly significant differences ( < 0.001; test). Number of replicates ≥6.