Quercetin Increases Hepatic Homocysteine Remethylation and Transsulfuration in Rats Fed a Methionine-Enriched Diet

Table 1

The primers used in real-time PCR.

Gene	GenBank		Primers
MS	NM_030864.1	F	5′-GGATCTCTGGGTCCGACTAACAA-3′
		R	5′-GCCTGCTCCTGGTATGCTTCA-3′
BHMT	NM_030850.1	F	5′-GAACCAGAGTTGCCACCAGATG-3′
		R	5′-AGCAGCCGCCAATGTACCTGAC-3′
CBS	NM_012522.2	F	5′-AAGGCTGCCCAGGAGCTAAG-3′
		R	5′-CAGCATCCATTTGTCACTCAAGAAC-3′
CSE	NM_017074.1	F	5′-GTCCACAAACACAAAGACATCA-3′
		R	5′-AGGTCATCGGAAGTAACAGACA-3′
β-actin	NM_007393.1	F	5′-GTCCCTCACCCTCCCAAAA-3′
		R	5′-GCTGCCTCAACACCTCAACCC-3′

MS, methionine synthase; BHMT, betaine-homocysteine methyltransferase; CBS, cystathionine β-synthase; CSE, cystathionine γ-lyase; F, forward; R, reverse.