Research Article
A Novel Aqueous Micellar Two-Phase System Composed of Surfactant and Sorbitol for Purification of Pectinase Enzyme from Psidium guajava and Recycling Phase Components
Figure 3
Influence of pH on yield and purification factor partitioning of pectinase in ATPS. Effect of various pH on the partitioning of pectinase in top phase was investigated. Triton X-100/sorbitol with 54.2% TLL and 20% crude load was used in this experiment. The purification factor and yield were determined according to (5) and (6), respectively.