A Novel Aqueous Micellar Two-Phase System Composed of Surfactant and Sorbitol for Purification of Pectinase Enzyme from Psidium guajava and Recycling Phase Components

Table 1

Effects of various phase compositions on the pectinase activity of Psidium guajava.

Phase composition	Concentration (%, w/w)	Pectinase activity
Triton X-100	20	100.1 ± 0.33^a
	40	101.1 ± 0.02^a
	60	120.4 ± 0.11^b
	80	102.2 ± 0.01^c
Tween-80	20	92.3 ± 0.11^a
	40	91.1 ± 0.21^b
	60	102.4 ± 0.22^c
	80	98.6 ± 0.57^ab
SDS	20	62.3 ± 0.32^a
	40	71.2 ± 1.42^b
	60	62.2 ± 0.09^a
	80	61.1 ± 0.32^a
Sorbitol	20	122.1 ± 0.07^a
	40	108.6 ± 1.10^b
	60	101 ± 0.22^ab
	80	95.2 ± 0.52^c

The sample sizes for all experiments were three. ^a–dMean values followed by different letters differ significantly (p < 0.05).